Product Info Summary
SKU: | A12993-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PHF15/JADE2 Antibody Picoband™
SKU/Catalog Number
A12993-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PHF15/JADE2 Antibody Picoband™ catalog # A12993-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PHF15/JADE2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12993-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived mouse Cd14 recombinant protein (Position: D36-A260). Mouse Cd14 shares 68.2% and 85.7% amino acid (aa) sequence identity with human and rat CD14, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A12993-1 is reactive to JADE2 in Human
Applications
A12993-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
87.466kDa
Background of JADE2
PHF15, also known as Protein Jade-2, has 3 isoforms, a 790 amino acid isoform that is 87 kDa, a 789 amino acid isoform that is 87 kDa and a 562 amino acid isoform that is 63 kDa, has a histone H4-specific acetyltransferase activity as a component of the HBO1complex, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. This protein has been shown an interaction with ING4, KAT7, ING5, PACSIN, ZBTB3, ZBTB5, ZNF496, and VHL proteins in histone H3 acetylation, histone H4-K5 acetylation, histone H4-K8 acetylation, and histone H4-K12 acetylation biological processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PHF15/JADE2 using anti-PHF15/JADE2 antibody (A12993-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF15/JADE2 antigen affinity purified polyclonal antibody (Catalog # A12993-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF15/JADE2 at approximately 120 kDa. The expected band size for PHF15/JADE2 is at 87 kDa.
Protein Target Info & Infographic
Gene/Protein Information For JADE2 (Source: Uniprot.org, NCBI)
Gene Name
JADE2
Full Name
E3 ubiquitin-protein ligase Jade-2
Weight
87.466kDa
Superfamily
JADE family
Alternative Names
E3 ubiquitin-protein ligase Jade-2 JADE2 JADE-2, PHF15 jade family PHD finger 2 E3 ubiquitin-protein ligase Jade-2|PHD finger protein 15|jade family PHD finger protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JADE2, check out the JADE2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JADE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHF15/JADE2 Antibody Picoband™ (A12993-1)
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No publications found for A12993-1
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Customer Reviews
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